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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Hematology20-25%- Hemostasis and coagulation testing
- White blood cell morphology and diseases
- Routine and automated hematology procedures
- Red blood cell physiology and disorders
Clinical Chemistry20-25%- Carbohydrates, lipids, proteins, and enzymes
- Endocrinology and therapeutic drug monitoring
- Toxicology and liver/kidney function tests
- Electrolytes, acid-base balance, and blood gases
Microbiology15-20%- Identification of pathogenic microorganisms
- Mycology, parasitology, and virology
- Specimen collection and culture techniques
- Bacteriology and antimicrobial susceptibility
Urinalysis and Other Body Fluids5-10%- Cerebrospinal, serous, and synovial fluids
- Physical, chemical, and microscopic analysis
- Semen and amniotic fluid testing
- Urine formation and composition
Immunology and Serology5-10%- Autoimmune and immunodeficiency disorders
- Infectious disease serology
- Immune system principles and components
- Immunologic testing methodologies
Blood Banking (Immunohematology)15-20%- Blood group systems and antigens
- Compatibility testing
- Blood components and storage
- Transfusion reactions and adverse events
- Antibody detection and identification
Laboratory Operations5-10%- Quality control and quality assurance
- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance
- Laboratory mathematics and instrumentation

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. The purpose of protective isolation is to protect:

A) the phlebotomist after a needlestick
B) the patient from family conflicts
C) a compromised patient from infection
D) the phlebotomist from infection


2. The risk of HbS polymerization is enhanced by a low (acid) pH, a state of dehydration, and increased levels of
2,3-DPG. Increased temperature (above 37°C) also adds to the risk.
Hematology
Which group of conditions INCREASES the risk of HbS polymerization?

A) Alkaline pH, dehydration, increased level of 2,3-DPG
B) Acid pH,dehydration, increased level of 2,3-DPG
C) Alkaline pH,dehydration, decreased level of 2,3-DPG
D) Acid pH, dehydration, decreased level of 2,3-DPG


3. The Bethesda assay is used to measure the titer and activity of the antibody present in a patient's sample.
Prothrombin time is an initial screening procedure for bleeding disorders and a test used for monitoring anticoagulant therapy. A thrombin time is used to detect heparin interference in an aPTT mixing study. A mixing study is performed to detect the presence of a factor deficiency or coagulation inhibitor, but does not quantify the result.
Hematology
Which of the following tests is used to quantify a coagulation inhibitor?

A) Mixing study
B) Prothrombin time
C) Bethesda assay
D) Thrombin time


4. The hepatitis B vaccine is a series of immunizations consisting of three injections of the hepatitis B antigen.
The antigen causes the recipient of the injection to make antibodies against the antigen, hence why this is an example of active immunity.
Immunology
The hepatitis B virus vaccine was administered to MLS students during Orientation. Which type of immunity is expected to develop and provide long-term protection?

A) inactive
B) adoptive
C) innate
D) passive
E) active


5. Alkaline over acid, or K/A, in TSI reactions is associated with the fermatation of glucose alone and the utilization of peptone.
Which of the following sugars has been fermented by a gram-negative rod that has produced an alkaline slant and an acid butt on triple sugar iron agar (TSI).?

A) sucrose
B) glucose and sucrose
C) glucose
D) lactose


Solutions:

Question # 1
Answer: C
Question # 2
Answer: B
Question # 3
Answer: A
Question # 4
Answer: E
Question # 5
Answer: C

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